Polarity sorting of axonal microtubules: a computational study
نویسندگان
چکیده
منابع مشابه
Polarity sorting of axonal microtubules: a computational study
We present a computational model to test a "polarity sorting" mechanism for microtubule (MT) organization in developing axons. We simulate the motor-based axonal transport of short MTs to test the hypothesis that immobilized cytoplasmic dynein motors transport short MTs with their plus ends leading, so "mal-oriented" MTs with minus-end-out are transported toward the cell body while "correctly" ...
متن کاملCytoplasmic Dynein Transports Axonal Microtubules in a Polarity-Sorting Manner.
Axonal microtubules are predominantly organized into a plus-end-out pattern. Here, we tested both experimentally and with computational modeling whether a motor-based polarity-sorting mechanism can explain this microtubule pattern. The posited mechanism centers on cytoplasmic dynein transporting plus-end-out and minus-end-out microtubules into and out of the axon, respectively. When cytoplasmic...
متن کاملPolarity orientation of axonal microtubules
The polarity orientation of cellular microtubules is widely regarded to be important in understanding the control of microtubule assembly and microtubule-based motility in vivo. We have used a modification of the method of Heidemann and McIntosh (Nature (Lond.). 286:517-519) to determine the polarity orientation of axonal microtubules in postganglionic sympathetic fibers of the cat. In fibers f...
متن کاملThe transport properties of axonal microtubules establish their polarity orientation
It is well established that axonal microtubules (MTs) are uniformly oriented with their plus ends distal to the neuronal cell body (Heidemann, S. R., J. M. Landers, and M. A. Hamborg. 1981. J. Cell Biol. 91:661-665). However, the mechanisms by which these MTs achieve their uniform polarity orientation are unknown. Current models for axon growth differ with regard to the contributions of MT asse...
متن کاملStructural Polarity of Kinetochore Microtubules
The polarity of kinetochore microtubules (MTs) has been studied in lysed PtK1 cells by polymerizing hook-shapdd sheets of neurotubulin onto the walls of preexisting cellular MTs in a fashion that reveals their structural polarity . Three different approaches are presented here : (a) we have screened the polarity of all MTs in a given spindle cross section taken from the region between the kinet...
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ژورنال
عنوان ژورنال: Molecular Biology of the Cell
سال: 2017
ISSN: 1059-1524,1939-4586
DOI: 10.1091/mbc.e17-06-0380